RY, I would say. . .use the tubes you get with the amps for hundreds of hours. No need to really roll tubes until the amplifier is seasoned in. Personally I'm not a fan of the Cryotone tubes, the output tubes are good, the rectifiers my least favorite in their line, and the input and driver tubes are good. . .  I just in general prefer a different sound and presentation than the Cryotone's give, and have stopped using those that I had and use NOS and Chinese tubes now instead.

Below is my experience after over two years with the amp. I have done a lot of experimenting but as always it's my room and tastes reflected in these choices. Other owners will hopefully weigh in with other experiences.

First off: you are correctly identifying the driver and voltage regulation tubes.

I do have a lot of exprerience with the Sophia Electric Aqua II, but not the Aqua III. I did experience a few glitches with the Aqua II in the amp, and so moved on to other rectifiers. I found heaven with the Psvane 274B-Z--that's my favorite tube, which I use with both Shuguang Black Treasure 300B-Z output tubes which I really do like, and also with Linlai Dream Series 7300B-D output tubes, which are very interesting tubes, more "analytical" sounding to me than the Black Treasures, and good for some listening sessions with some material. I also occasionally roll in another rectifier with either output tube pairs, a Linlai Dream Series 274B-D which is a great match to the 7300B-D and also give a bit of "pep in the step" to the Black Treasures.

For input tubes after a lot of rolling I have settled on Holland made 12AU7 as the input tube, but as you have a stock of 12AU7 when the amp is seasoned try what you have. And for driver tubes I've moved to French 7308 type tubes with slanted dimpled getters. . . these are a tricky match, but with the other tubes in this complement a very good one. For the voltage regulation tubes I have tried many combinations and keep coming back to English made 75C1 tubes, dark coated small tubes presenting with lots of texture and richness.

It's hard to make this amp sound bad, and with different tube complements can sound like a different amplifier. . . it's almost as if there is a multiverse of amplifiers in this one chassis. You'll have fun exploring after it has seasoned in and taught you what gain structure etc. you and your room like.

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Yeah, right . Everyone has a different "best." I'd encourage you to read the threads here, and in the Reviews and 300B fora to get an idea of what other people like and what they don't. Spoiler, some people will like and others will dislike pretty much any tube out there. But I wouldn't necessarily run out to buy a bunch of driver tubes tomorrow, see below.

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Short answer, probably yes. There are actually two different burn ins you need to consider. The first is the amp, do you know (approximately) how many hours it has on it? A brand new Decware amp can sound pretty funky for the first hundred or so hours, after that the changes and improvement are pretty slow and gradual. A bigger thing in my experience is the 300B tubes can take hundreds of hours to burn in. I had bargain Chinese 300Bs that took about 200 hours to sound their best, and Japanese 300Bs that took fully 500. So, I would definitely give it a while before you start seriously comparing the SQ. Another thing you will find by reading other posters experience, with the 300B and other amps, is a number of them are underwhelmed on first (and often second and third) listen, but after a few days/weeks/whatever they realize how great Decware amps sound. Good luck with your journey and keep us posted with your progress.

Interesting that the Cryotone tubes were bad. I’ve had the same Cryotones in my UFO for 4-ish years now, literally thousands of hours, and still going strong. I had an issue with some Cryotone rectifiers not playing nicely with my SEWE300b but never the drivers.

Anyway, welcome to the forums and your new addiction. Glad the new amp is blossoming- it will continue to get better and better.